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Fig. 2 | Zoological Letters

Fig. 2

From: Targeted deletion of liver-expressed Choriogenin L results in the production of soft eggs and infertility in medaka, Oryzias latipes

Fig. 2

RT-PCR analysis of chg.l+/+, chg.l+/−, and chg.l−/− medaka. A The position of the primers used for the analysis. B The result of RT-PCR analysis of chg.l+/− and chg.l−/− medaka. Abbreviations: M – marker; G – genomic DNA extracted from the chg.l+/+ fin with sodium hydroxide; H – chg.l+/− (del-14 heterozygous knockout (KO)); N – chg.l−/− (del-14 homozygous KO). The targeted genes for the RT-PCR assay, primers, size of the cDNA product, and anticipated size of the genomic DNA are summarized in Supplemental Table S2. The primer Pairs for PCR analysis included (1) Chg. L FW1 and Chg. L RV2, (2) Chg. L FW2-del and Chg. L RV2, (3) Chg. H Ex1 FW1 and Chg. H Ex2 RV1, (4) Chg. Hm Ex1 FW1 and Chg. Hm Ex2 RV1, and (5) Chg. Hm Ex1 FW1 and Chg. Hm Ex3 RV1

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Zoological Letters

ISSN: 2056-306X

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