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Fig. 3 | Zoological Letters

Fig. 3

From: Targeted deletion of liver-expressed Choriogenin L results in the production of soft eggs and infertility in medaka, Oryzias latipes

Fig. 3

Spawning females, ovaries, and oocytes of chg.l+/+ and chg.l−/− medaka. Panel A Spawning females, dissected ovaries, and ovulated eggs of chg.l+/+ and chg.l−/− medaka. Aa and Ab chg.l+/+ and chg.l−/− females, respectively, with a yellow circle highlighting spawned eggs in from the former and a red bracket highlighting spawned string-like materials containing “smashed eggs” from the latter. Ac and Ad Isolated ovary and ovulated eggs from the chg.l+/+ female. Ae and Af Isolated ovary and ovulated eggs from the chg.l−/− female. Panel B Changes in the artificially inseminated eggs. Ba and Bb chg.l +/+ and chg.l −/− ovaries, respectively, used to obtain ovulated and fully-grown oocytes (white arrows) for this “in vitro fertilization” experiment. Bc and Bd chg.l+/+ and chg.l−/− eggs, respectively, immediately before insemination. Be and Bf-g chg.l+/+ and chg.l−/− eggs, respectively, at 20 min post-insemination (mpi). Bh and Bi-j chg.l+/+ and chg.l−/− eggs, respectively, at 60 mpi, Bk and Bl chg.l+/+ and chg.l−/− eggs, respectively, at 360 mpi, Bm chg.l+/+ eggs at 120 h post-insemination (hpi). White arrowheads show the perivitelline space in Be, Bh, and Bk. Yellow arrowheads show oil droplets in Bc-h and Bj. Yellow arrows show developing embryos in Bk and Bm. Red arrows show white turbidity, such as emulsions in Bf, Bi, and Bl. Scale bars in Aa and Ab = 1 cm; in Ac-f and Ba-b = 1 mm; and in Bc-l = 5 mm

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