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Fig. 5 | Zoological Letters

Fig. 5

From: Targeted deletion of liver-expressed Choriogenin L results in the production of soft eggs and infertility in medaka, Oryzias latipes

Fig. 5

Western blot analysis and IHC detection of egg envelope proteins in the ovaries of chg.l+/+ and chg.l−/− females using specific antibodies against Chgs. Panel A SDS-PAGE results showing proteins stained with CBB (Aa-Ad) or immunoreactive proteins with anti-medaka Chg. L (Ae-Ah) or Chg. H (Ai-Al). The numbers on the left indicate the molecular weights of the proteins (blue bands) in kilodaltons (kDa). Symbols - Arrowheasds: the position of Chg. H and Chg. Hm (corresponding to ZI-1 and -2 as chorion components). Stars: the position of Chg. L (corresponding to ZI-3 as a chorion component). No immunoreactive proteins were observed in the negative controls for the Western blot assay (i.e., with primary antibody omitted; data not shown) or the experiment (i.e., the liver and blood plasma extracts from male fish; data not shown). Abbreviations - L: liver extracts. Ov: ovary extracts. Panel B Immunohistochemical detection of egg envelope proteins in the ovaries of the chg.l+/+ and chg.l−/− females with anti-Chg.L and anti-Chg. H antibodies. Ba, Be, and Bi Transmission light micrograph of the observed ovarian oocyte in the ovary of the chg.l+/+ female (Ba), ovarian egg in the ovary of the chg.l−/− female (Be), and magnified portion in Be circled with red (Bi). Bb, Bf, and Bj Immunoreactivity with anti-Chg.L antibodies in the same ovarian oocyte as shown in Ba, Be, and Bi, respectively. Bc, Bg, and Bk Immunoreactivity with anti-Chg.H antibodies in the same ovarian oocyte as Ba, Be, and Bi, respectively. Bd Transmission light micrograph (Ba) overlaid with Bb and Bc. Bh Transmission light micrograph (Be) overlaid with Bf and Bg. Bl Transmission light micrograph (Bi) overlaid with Bj and Bk. Symbol - White arrows: position of the chorion. Abbreviation - y: yolk. Scale bars represent 500 μm (Ba, Bb, Bc, Bd, Be, Bf, Bg, Bh) and 50 μm (Bi, Bj, Bk, Bl)

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