Fig. 4
From: Hedgehog signaling controls mouth opening in the amphioxus
Ptch expression in Smo mutant embryos. Ptch expression was visualized by in situ hybridization, and all embryos were placed anterior to the left; a, b: Ptch expression in WT or Smo+/− embryos; a1, b1: Ptch expression in Smo−/− embryos. Smo gene knockout has no effect on Ptch expression at the N1 stage but results in diminished Ptch expression at the T1 stage. N1, early neurula stage; T1, tail bud stage before mouth opening; scale bar, 100 μm