Table 1 List of primers used in this study
From: Hedgehog signaling controls mouth opening in the amphioxus
Name of gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Restriction site |
|---|---|---|---|
Smo | GGTACCTTTCCACCATGTTGAGGAGCG | ACTAGTGGTTCTTCACAGTACTCTGTATC | KpnI/SpeI |
Cer | GGTACCATGAAGACGAGCGTGAGGAGC | ACTAGTTCAGAAGTACTTATCCCCACATG | KpnI/SpeI |
Nodal | GGTACCGCAGGCCGAGACCAACACCGC | ACTAGTCTACTGACAGCCGCATTCATCC | KpnI/SpeI |
Lefty | CTCGAGTACGATGAAACCTGTTCTAGTT | ACTAGTTTACTGTGTGCACGCACACTG | XhoI/SpeI |
Pitx | GGTACCACATATCTAAGGAGGACATCGTG | ACTAGTTCTTTAGCAAACAAATCCCATACGC | KpnI/SpeI |
Ptch | ACGGTTGGACATATTCTGTTGC | TGATACCATCCGCTCATTTCTG | NA |
Pou4 | GGTACCAGAACAGATGATGAACGGGAAAC | ACTAGTTTGGGCGGTGCGATAGTAGAG | KpnI/SpeI |
Pax2/5/8 | ATGGACAGGATGACCACGATG | GTGAGAAGAGAAGAAGTTGCC | NA |
Frzb1 | GGTACCGCGATATTGAATTTAGCGTGGT | ACTAGTCGAGTTGTCAGGGTCTTAGCA | KpnI/SpeI |