Fig. 4

Overall views of gene expression patterns in the olfactory organ of bichir. (A-E) The expression patterns of four VNO-related genes (ancV1R, TRPC2, Gi₂, Go) and MOE-related genes (Golf) in the olfactory lamellae. (A’-E’) Higher magnification views of the MOO in the solid squares in (A-E). (A”-E”) Higher magnification views of the lamellae of the AOO in the dotted squares in (A-E). Note that ancV1R, TRPC2, Gi₂, and Go are expressed in the basal layer, while Golf is expressed in the apical layer of the lamellae. (F-H) Coexpression of ancV1R (magenta) with (F) TRPC2, (G) Gi2, and (H) Go (green). Arrowheads indicate the coexpressing cells. (I) Contrasting expression patterns of Go (magenta) in the basal layer and Golf (green) in the apical layer of the MOO lamellae confirmed by two-color FISH. The cell nuclei were stained by DAPI (blue). The dotted line indicates the center of the lamellae. The scale bars indicate 500 μm (A-E), 50 μm (A’-E’, A”-E”, I), or 20 μm (F-H)