Fig. 6

Impaired transformation of dermal fibroblasts into cartilage cells by inhibition of Pde4b function by Rolipram. A Cyclic AMP concentration in limb tissues was measured by ELISA. B The schematic diagram of the experiment. C Rolipram treated limb in the bright-field view (C1), the dark field view (C2), and the merged image (C3). Scale bar in C1 = 3 mm. D, E Distribution of the GFP⁺ cells in the regenerated limbs in the control (D) and the Rolipram treated animal (E). Col2a1 expression was visualized I by in situ hybridization (D1, E1) and GFP signals were detected by immunofluorescence (D2, E2) on the identical sections. D3 and E3 were the highly magnified and merged images. The scale bar in D2 and D3 are 300 μm and 100 μm, respectively