Fig. 7

Distribution of GFP+ cells in the dibutyryl-cAMP treated animal. A the schematic diagram of the experiment. The GFP-skin grafted limb of the control animal (B–E) and the dibutyryl-cAMP treated animal (F–I). B, F The GFP-grafted limb just before sampling. C–E, G–I The distribution of the GFP⁺ cells and Col2a expression were visualized by immunofluorescence. E, I the merged and higher magnified image of C and D. The asterisks in I indicate the red blood cells. The dotted lines indicate the border of the cartilage. J The plot of the number of GFP⁺Col2a⁺ cells. The scale bars in B1, C, and E are 3 mm, 300 μm, and 100 μm, respectively