Fig. 8

Inhibition of the participation of dermis-derived newt cells into cartilage by Rolipram treatment. A the schematic diagram. B Rolipram treatment inhibited the formation of cell aggregate by B2FF application. C–E Cell morphology 2 weeks after the treatment. C the control. D The B2Ff treated cell. The aggregate was formed. E The Rolipram+B2FF treated cells. No cell aggregate could be seen. F–J Distribution of the grafted newt cells in axolotl limb regeneration. F The newt cell-grafted limb regenerates normally. G The grafted newt cells (red). H–J The distribution of the grafted newt cells and cartilages was visualized by immunofluorescence. J the high magnified and merged image of the H and I. Nucleai are shown in blue (Hoechst). K the plot of the number of the Col2A⁺mCherry⁺ cells. The scale bars in C, F, and H are 500 μm, 2 mm, and 300 μm, respectively