Fig. 9
From: An approach for elucidating dermal fibroblast dedifferentiation in amphibian limb regeneration
Transdifferentiation from the dermis to cartilage in the mosaic Pde4b crispants. A–C the control animals (wt: wild-type). D–E The mosaic Pde4b crispants. A, D limb morphology. B, E The distribution of the GFP+ cells. GFP plasmids were electroporated in the dermal region prior to amputation. C, F the merged images of the Col2a1, GFP, and the bright field view. Col2a1 and GFP were visualized by in situ hybridization and immunofluorescence, respectively. The scale bars in A and C are 1 mm and 300 μm, respectively. G the plot of the GFP + Col2a1+/GFP+ ratio along with the KO score calculated by ICE analysis