Table 3 Primers used in qPCR of goldfish Cry genes
Gene | Primer | Sequence (5’ to 3’) | Amp. Eff. (%) |
|---|---|---|---|
gCry1aa | Forward | TGGCC TGGAG GAGAA ACAGA | 94.2 |
Reverse | CAACA GCGAA TTGGC ATTCA | ||
gCry1ab | Forward | CAGTG TCATG TGGTC AACTG TCTG | 101.0 |
Reverse | TGTCC CTCTCC CTCTC TGTGA | ||
gCry1ba | Forward | TTTAC CGCGG GAAGA GATGA | 90.6 |
Reverse | TGCAG GGTTG TCATG GAGAC | ||
gCry1bb | Forward | CGCCT GAACA TCGAG AGGAT | 94.3 |
Reverse | CCCAC GACCT CCATG TGATT | ||
gCry2 | Forward | GGCAC AGGAA TATGG TGTGG A | 95.2 |
Reverse | GAGTC GATTC ACAAT GGCTT GA | ||
gCry4 | Forward | CGCAT TCTTC CACAA ATACA CC | 95.0 |
Reverse | ACGTC TTCTG GAGCT TTCCA C | ||
gGusb | Forward | ACCGG GAACC ATCCA GTACA | 96.3 |
Reverse | CGATC GGTGT ATTCC AGCGT A | ||
gPgk1 | Forward | CGTTG GACAA GGTGG ATGTG | 101.7 |
Reverse | TGATG GAACT GCAGC CTTGA | ||
gHprt1 | Forward | AAGTG GCCAG TTTGC TGGTG | 104.5 |
Reverse | GTCAA GTGCA TATCC AACCA CA |