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Table 3 Primers used in qPCR of goldfish Cry genes

From: Midnight/midday-synchronized expression of cryptochrome genes in the eyes of three teleost species, zebrafish, goldfish, and medaka

Gene Primer Sequence (5’ to 3’) Amp. Eff. (%)
gCry1aa Forward TGGCC TGGAG GAGAA ACAGA 94.2
Reverse CAACA GCGAA TTGGC ATTCA
gCry1ab Forward CAGTG TCATG TGGTC AACTG TCTG 101.0
Reverse TGTCC CTCTCC CTCTC TGTGA
gCry1ba Forward TTTAC CGCGG GAAGA GATGA 90.6
Reverse TGCAG GGTTG TCATG GAGAC
gCry1bb Forward CGCCT GAACA TCGAG AGGAT 94.3
Reverse CCCAC GACCT CCATG TGATT
gCry2 Forward GGCAC AGGAA TATGG TGTGG A 95.2
Reverse GAGTC GATTC ACAAT GGCTT GA
gCry4 Forward CGCAT TCTTC CACAA ATACA CC 95.0
Reverse ACGTC TTCTG GAGCT TTCCA C
gGusb Forward ACCGG GAACC ATCCA GTACA 96.3
Reverse CGATC GGTGT ATTCC AGCGT A
gPgk1 Forward CGTTG GACAA GGTGG ATGTG 101.7
Reverse TGATG GAACT GCAGC CTTGA
gHprt1 Forward AAGTG GCCAG TTTGC TGGTG 104.5
Reverse GTCAA GTGCA TATCC AACCA CA