Fig. 7

Detection and localization of mZPC5 proteins in chg.l -/- females. A: SDS-PAGE and western blot assays of the liver (7Aa and 7Ab) and ovary extracts (7Ac and 7Ad) using anti-mZPC5 antibody. 7Aa: the 10% SDS-PAGE pattern of proteins in the liver extracts stained with CBB. Ab: proteins immunoreactive to the anti-mZPC5 antibody in the liver extracts. Ac: 8% SDS-PAGE pattern of proteins in the ovary extract stained with CBB. Ad: proteins immunoreactive to the anti-mZPC5 antibodies in the ovary extracts. B–D: confocal microscope observations of the same ovarian oocytes (ovarian stage less than V) to identify the locations of immunoreactive proteins in the ovarian oocytes using anti-mZPC5 antibodies as probes. B: a transmitted light image provided to illustrate the exact morphology of the ovarian oocytes without immunochemical staining. C: same as B but including the localized anti-mZPC5-immunoreactive proteins. D: overlaid images B and C. Symbols: yellow star and black arrow in Ad – immunoreactive proteins with 72–72 kDa and 50 kDa molecular masses, respectively; white arrows in C and D – oocytes that were immunoreactive to anti-mZPC5 antibodies. Scale bar in Panel B = 100 µm