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Fig. 8 | Zoological Letters

Fig. 8

From: Medaka, Oryzias latipes, egg envelopes are created by ovarian-expressed ZP proteins and liver-expressed choriogenins

Fig. 8

Immunohistochemical detection of mZPC5 in a chg.l-/- female ovary. 8A and 8E: Transmitted light image of an ovarian oocyte in the ovary of a sexually mature female. 8B and 8F: The same ovarian oocytes shown in 8A and E, respectively, but with highlighted issue regions immunoreactive to anti-mZPC5 antibodies. 8C and 8G: The same ovarian oocytes shown in 8A and 8E, respectively, but with highlighted issue regions immunoreactive to anti-Chg.H antibodies. 8D: Transmitted light image 8A overlaid with 8B and 8C. 8H: Transmitted light image 8E overlaid with 8F and 8G. White arrows: position of the chorion. It should be noted that 8E to 8H show magnifications of the white-circled area in 8A. Symbols – white stars: the location of ZPC5 in the ooplasm. Scale bars in 8A and 8E represent 100 and 20 µm, respectively. The developing stage of the oocytes was determined following Iwamatsu et al. 1988 [34]

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Zoological Letters

ISSN: 2056-306X

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