Fig. 2
From: Air–breathing behavior underlies the cell death in limbs of Rana pirica tadpoles
Distribution of cell death and ROS during limb development and experimental conditions in R. pirica. a Cell death (LysoTracker Green) and ROS (CellROX) staining of late stage 36 R. pirica hindlimbs. The arrowheads point to cells positive for both LysoTracker and CellROX staining. The asterisks are microbes, not stained cells. b Cell death (LysoTracker Red) in late stage 36 R. pirica hindlimbs incubated for 3 hours under normoxia or hyperoxia. c Quantification of LysoTracker–positive cells in ID4 of the hindlimbs indicated in (b). Each symbol represents an individual specimen. Mean ± SEM. Two–tailed unpaired t–test. ∗p < 0.05. ID3–4: interdigital regions 3 and 4. Scale bar, 100 μm in (a) and 500 μm in (b)