Fig. 6

Drawing and CLSM of the acetylated α-tubulin-LIR nervous system of Diuronotus aspetos. Anterior pointing left for a–i, and pointing at the top for j and k. a, b Schematic drawings of the α-tubulin-LIR of the anterior part of the specimen: nerves in blue, nuclei in grey, and opposite ventral or dorsal nervous system in light grey a dorsal b ventral. c CLSM ventral view of the maximum intensity projection (MIP) of the entire specimen. d–k CLSM MIP sub-stacks of various parts of the specimen. Acetylated α-tubulin-LIR in glow, and DAPI in cyan in all CLSM pictures. d Ventro-anterior nervous system. e Neuropil side f Ventral, post pharyngeal ganglion. g Ventral, trunk commissure. h Dorso-posterior part of the head i ventro-anterior part of the head j Dorso-anterior part of the head. k Ventro posterior terminal part of the specimen. ang anal ganglion, anr anterior nerve ring, avmn anterior ventro-median nerve, br brain, cpn ciliated patch nerves, danp dorso-median anterior nerve projection, dlnp dorso-lateral anterior nerve projections, hdpn head dorso-posterior nerve, hdn head diagonal nerve, hln head lateral nerve, lgcb lateral gland cell of the brain, np neuropile, nppt nerve projection of the primary tube, pac pre-anal commissure, pco posterior commissure, pgg post-pharyngeal ganglion, ph pharynx, spc sub-pharyngeal commissure, tt, testis, tvc trunk ventral commissure, vlnc ventro-lateral nerve cord