Fig. 8

FMRF-amide-LIR nervous system of Diuronotus aspetos. Anterior is pointing left for a–g and i–l and dorsal pointing at the top for h. a, b Schematic drawings of the FMRF-amide-LIR of the anterior part of the specimen: nerves in magenta, nuclei in grey, and opposite ventral or dorsal nervous system in light grey. a Dorsal view, b ventral view. c CLSM dorsal view of the maximum intensity projection (MIP) of the entire specimen. d–l (Except h) CLSM sub-stack MIP of various parts of the specimen. FMRF-amide-LIR in glow, and DAPI in cyan in all CLSM pictures. d Dorsal view of the whole neuropil. e Ventral part of the brain. f and g different levels of the dorsal part of the neuropil. h CLSM virtual transverse section of the anterior nerve ring. i Ventro-anterior part of the head. j Ventral commissure of the anterior nerve ring. k Ventral post-pharyngeal ganglia. l ventro-posterior terminal part of the specimen. Anterior of the specimen on the left for a-g and i-l and dorsal on top for h. br brain, egg egg, fanr FMRF-amide-LI-reactive anterior nerve ring, fapn FMRF-amide-LI-reactive anterior perikarya of the ventro-lateral nerve cord, fdpc FMRF-amide-LI-reactive dorso-posterior cluster of the brain, flnc FMRF-amide-LI-reactive ventro-lateral nerve cord, flpb FMRF-amide-LI-reactive lateral perikarya of the brain, fnp FMRF-amide-LI-reactive neuropil, fnpt FMRF-amide-LI-reactive nerve projection of the primary tube, fpar FMRF-amide-LI-reactive dorso-median perikarya of the anterior nerve ring, fpco FMRF-amide-LI-reactive posterior commissure, fpp FMRF-amide-LI-reactive perikarya of the dorso-lateral anterior nerve projections, fppg FMRF-amide-LI-reactive post-pharyngeal ganglion, fspc FMRF-amide-LI-reactive sub-pharyngeal commissure, fvmn FMRF-amide-LI-reactive anterior ventro-median nerve, fvnc FMRF-amide-LI-reactive anterior ventro-median nerve cluster, fvpb FMRF-amide-LI-reactive ventro-lateral perikarya of the brain, fvpr FMRF-amide-LI-reactive ventral perikarya of the anterior nerve ring, ph pharynx