Table 3 Primers used for qPCR analyses of candidate genes.
Target gene | Accession # | Forward Primer 5' to 3' | Reverse Primer 5' to 3' | product size, bp | Ta, °C |
|---|---|---|---|---|---|
cyp11a1 | NM_152953 | AAAGCCTGAAGACGGTGCTA | AGCAGGACGCCATATTTTTG | 117 | 60 |
zgc:136308 | NM_001111227 | GCAAACACGACACAACTCCTGC | TGTGTCCTCCATCAGGTCTGTTTAC | 130 | 56 |
efna1 | NM_200783 | AGCAGTTGGCGAAGGTGATG | CGGTAATGGAGGAGGCGTTC | 99 | 56 |
slc37a4a | NM_214738 | CTCCAGCAAAAATGAAAGCA | CACCCCAAACACCACCAG | 91 | 56 |
Slc25a10 | NM_201172 | TAATATACTCACACACTTCCTG | CTGTATTCTCCTTTAGAGTTC | 114 | 56 |
Spata | NM_001018678 | AGAAATAACACTCAAAGAGG | AGACTACAAGGAGAAACACT | 174 | 55 |
sox21a | NM_131286 | GTTCCCTCATCTTATGTACT | TTAAACTCCACTCATATCGT | 92 | 56 |
api5 | NM_199540 | GAGTCAAATCTTACCTTTCA | ACATACATCAGGGCATAATA | 86 | - |
nup85 | NM_001003625 | CACTCTTACAGACCATGCCCATATT | CCAGTGTCTCCATTTCACATCAAAC | 80 | 60 |
gbx2 | AF288762 | CATTAACACAACCATAATCC | GTCACTAACACAGTCTCACAT | 106 | - |
zgc65811 | NM_200552 | TCCTCATGTTAGTTTAAGGTCACGG | ACAGAAATGAAAGAGAAGCAGAAGT | 90 | 60 |
cnn2 | NM_213349 | GGACTACAGATGGGAACAAATAAAT | ATGTGTGACTTGGGATAATACAGAT | 92 | 60 |
CK139976 | CK139976 | CAGTATCTGCGATGTTTAATGTCAG | GCTGTCTGAGTCTTCCATTTGA | 102 | 61 |
bcl3 | XM_688922 | TGAAGAAAGAGGTGTGAGTTGATAG | TTAAAGAGACACAATGCTGAACGAA | 115 | 61 |
asic2 | NM_214788 | AATGTTCTTTGAGGATGGATGGTT | GTGACCTTGTATTTAGATTGAGAGC | 80 | 60 |
zgc:56525 | NM_200279 | CAACATTTCCTCCAGTGCTAAAG | CTTCAGTTCACTCTGCATCTTATTC | 94 | 55 |
asic4b | NM_214786 | GAGGAGTACATCAGAGACAACTTTC | CTTTCTTCTGCTCAATCGTTTCATA | 82 | 57 |
rhbdl3 | NM_001017556 | TCACTATAACAGAGGTTGTTGTCTT | TTCAGGAAGTATGGCGATGATAC | 88 | - |
LOC100003798 | XM_001343224.1 | GTACCTCAGTCAATCTCTAATCCTC | GAACCTTTGTTCATCTCTTCTGTTT | 85 | 60 |
nptna | NM_001160156 | ATACAGGCATTTCCAGGCTTTATTT | AAAGAACTGTCCAACCAGAATCAT | 110 | 60 |
zgc:92184 | NM_001002344 | CCCGTGGCGGCGATATGCTT | TCCCCCGCAGCGTCTGATGA | 544 | 56 |
ptgs2a | NM_153657 | ATGTTTGCTTTCTTCGCCCA | AGATCCACTCCATGACCCAG | 101 | 55 |
ef1α | L47669 | CTTCTCAGGCTGACTGTGC | CCGCTAGCATTACCCTCC | 358 | 60 |