Skip to main content

Table 1 Otolith formation in MO or mRNA of OlPKS

From: Unexpected link between polyketide synthase and calcium carbonate biomineralization

Experiment

Nucleltide

Fish

4 otoliths

1-3 otoliths

No otolith

n

Phenocopy

olpks first-Met MO

wt

0%

0%

100%

39

Rescue

(uninjected)

ha

0%

0%

100%

31

olpks mRNA

73%

23%

3%

30

Active site mutation

(uninjected)

ki79

0%

0%

100%

14

olpks-mRNA

65%

35%

0%

43

KS*-mRNA

0%

0%

100%

15

AT*-mRNA

0%

2%

98%

57

DH*-mRNA

0%

0%

100%

18

KR*-mRNA

0%

0%

100%

17

ACP*-mRNA

0%

0%

100%

27

Loop*-mRNA

53%

40%

7%

60

  1. Asterisks indicate one amino acid mutation are introduced.
  2. ‘Loop’: interdomain region.
  3. Fully rescued: 4 otoliths in one animal.
  4. Partially rescued: 1–3 otoliths in one animal.
  5. Not rescued: no otolith.