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Table 1 Otolith formation in MO or mRNA of OlPKS

From: Unexpected link between polyketide synthase and calcium carbonate biomineralization

Experiment Nucleltide Fish 4 otoliths 1-3 otoliths No otolith n
Phenocopy olpks first-Met MO wt 0% 0% 100% 39
Rescue (uninjected) ha 0% 0% 100% 31
olpks mRNA 73% 23% 3% 30
Active site mutation (uninjected) ki79 0% 0% 100% 14
olpks-mRNA 65% 35% 0% 43
KS*-mRNA 0% 0% 100% 15
AT*-mRNA 0% 2% 98% 57
DH*-mRNA 0% 0% 100% 18
KR*-mRNA 0% 0% 100% 17
ACP*-mRNA 0% 0% 100% 27
Loop*-mRNA 53% 40% 7% 60
  1. Asterisks indicate one amino acid mutation are introduced.
  2. ‘Loop’: interdomain region.
  3. Fully rescued: 4 otoliths in one animal.
  4. Partially rescued: 1–3 otoliths in one animal.
  5. Not rescued: no otolith.