Fig. 4
From: Evidence for a cordal, not ganglionic, pattern of cephalopod brain neurogenesis
ELAV gene expression identifies neuronal somata in the brains of O. bimaculoides hatchlings. a–c Midline section taken through hatchling brain, with the mouth to the left. d–f High-power view of the transition between the rind of neuronal cell bodies and the neuropil core of the subesophageal mass. Section was double-stained for (a and d) anti-acetylated alpha-tubulin (acTUBA) and (b and e) ELAV gene expression. c and f In silico merging of fluorescence and light microscopic images. es, esophagus; subes, subesophageal mass; supes, supraesophageal mass. Scale bars: 100 μm (a) and 50 μm (d)