Fig. 9

Median sections of the tadpole brain. Neurons are visualized by immunohistochemistry (shown in green). a Dorsal view of the Xenopus larva that performed the fluorescent immunostaining. Dashed line indicates the cutting plane in (b–e). b’–e’ High magnification images of the box in (b–e): b, b’ Slit2-control-MO, c, c’ Slit2-MO, d, d’ Robo2-control-MO, e, e’ Robo2-MO. In the Slit2/Robo2-control-MO, the posterior commissures make a tight bundle, whereas in the Slit2/Robo2-MO injected larvae, the width of PC is elongated anteroposteriorly. Blue staining shows nucleus labeled by DAPI. Scale bars: 100 um