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Fig. 5 | Zoological Letters

Fig. 5

From: Distal-less induces elemental color patterns in Junonia butterfly wings

Fig. 5

Dll-induced ectopic color patterns. White arrows indicate ectopic patterns. Areas in green circles in the left panels are enlarged in subsequent panels. a-c Black and orange mosaic clusters of scales. Scale morphology and size are normal, but colors are not. Color quality of these modified scales is similar, if not identical, to that of the nearest eyespot. Scale bars: 5 mm (a), 1 mm (b), and 500 μm (c). d-f Orange and black (gray) mosaic cluster of scales. Colors are not as vivid as those in a-c, but the mosaic pattern is similar. Some scales show reversed direction, as indicated by yellow arrows and white broken line in f. Scale bars: 5 mm (d), 1 mm (e), and 500 μm (f). g-j Black and gray clusters of scales. Transparent scales are observed in an area superimposed on the large eyespot. The modified area is surrounded by white broken lines in i. The area with transparent scales is indicated by green broken lines and an asterisk in i, from which GFP fluorescent signal was obtained, as indicated in j. The GFP signal is observed in the basal membrane but not the scales themselves. Scale bars: 5 mm (g), 500 μm (h), and 200 μm (i, j). k-n GFP-positive rectangular cluster of gray scales. As shown in m and n, GFP signal is observed from the scales themselves. Scale bars: 5 mm (k), 500 μm (l, m), and 200 μm (n). o-q Black spots. The basal membrane but not scales shows GFP fluorescence. Scale bars: 5 mm (o) and 200 μm (p, q). r-u GFP-positive necrosis-like damage. A GFP-positive black patch is observed. In addition, gray bands that contain undeveloped scales are observed. Scale bars: 5 mm (r), 1 mm (s), and 200 μm (t, u)

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