Fig. 5

Levels of histone H3 trimethylated at K27 (H3K27me3) in the optic lobe of the cricket Gryllus bimaculatus. (a, b) location of H3K27 in the optic lobes under LD 12:12 (a) or LD 20:4 (b). The right three columns indicate high magnification images of the optic lobe indicated by square from the low-magnification image in the left column. All images are oriented with dorsal side at the top. Scale bar, 100 μm. Blue and green indicate nuclei (DAPI) and H3K27me3, respectively. (c) Staining indices of H3K27me3 in intact and Gb’E(z)^RNAi crickets in LD 12:12 and LD 20:4. Average of 6 optic lobes are shown with SD. Values with different lower case letters differ significantly from each other (ANOVA followed by Tukey-test, P < 0.05). Note that level of H3K27me3 is higher in LD 12:12 than LD 20:4 and is greatly reduced in Gb’E(z)^RNAi crickets