Fig. 7
From: Flexible selection of diversified Na+/K+-ATPase α-subunit isoforms for osmoregulation in teleosts
In situ hybridization of NKA α1c-1 (a, b) and immunohistochemistry of NKA protein (c, d) in the anterior intestine of eels acclimated to FW and 7 day SW. Positive mRNA signals (arrows) were localized at the mucosal epithelium. Strong and dense signals are present in SW-acclimated tissues. From immunohistochemistry, the NKA protein signals were observed at the basolateral side of the epithelium. Top right photos show the structure of the dotted square region in high resolution. Enclosed photos show the negative control of the hybridization incubated with sense probes. Arrow heads indicate the position of goblet cells (Gt). V = villus; M = muscle