Table 1 Primer sequences for real time PCR
From: Flexible selection of diversified Na+/K+-ATPase α-subunit isoforms for osmoregulation in teleosts
GenBank/Ensembl accession number | Gene & Primer names | Forward (F) Reverse (R) | Oligo sequences (5′ to 3′) |
|---|---|---|---|
Japanese eel | |||
KU976438 | atp1a1c-1 (α1c-1) | F | CTGTATGATGAAGCCCGAAGAT |
R | AATGTGTGATGCAGGCAGTA | ||
KU976439 | atp1a1c-2 (α1c-2) | F | GTACTACTGAAGCTTCCTGTCTG |
R | CACATCCAGAAGTTACTGAGGTTA | ||
KU976440 | atp1a1c-3 (α1c-3) | F | CCCTATTCACTCCTCATCTTCATT |
R | GCACTAGTAGTACGTCTCTCTCT | ||
KU976441 | atp1a2 (α2) | F | GACTCGACGCAACTCTGTTT |
R | ACGAAGATCAGGAGGCTGTA | ||
KU976442 | atp1a3 (α3) | F | CCTCATCTTTGTGTACGATGAGATA |
R | GATGGTGTGAAGCAGAAGAAATG | ||
AB593812 | eef1a (ef1α) | F | CTGAAGCCTGGTATGGTGGT |
R | ACGACGGATTTCCTTGACAG | ||
Medaka | |||
ENSORLG00000018557 | atp1a1a.4 (2 of 2) (α1a) | F | TAAATGAAAGGCTCATCAGTATGGC |
R | GTCTTCCAGGTCGTTTACAGG | ||
ENSORLG00000002122 | atp1a1a.4 (1 of 2) (α1b) | F | CTGATCTGCGAATTGTCTCCTC |
R | AGTCTGGGGTTCGGATTCAC | ||
ENSORLG00000002047 | atp1a1b (α1c) | F | CTGGCTGGATTCTTCACCTATT |
R | CCTCCAGGTCGTTGATGTATTT | ||
ENSORLG00000002639 | atp1a2a (α2) | F | TGCTGTGGGTAAATGCCGCTCT |
R | TTGGCTGTGATTGGGTGG | ||
ENSORLG00000007036 | atp1a3a (α3a) | F | GCTTATGAAGCAGCAGAAAGTG |
R | CCTATTTGTCCGTAGGCGATAC | ||
ENSORLG00000013191 | atp1a3b (α3b) | F | TGAGGAAATATGCAGGAAGTTGAAT |
R | CAGAAACTCTGCTGCCTTTGC | ||
ENSORLG00000007614 | eef1a (ef1α) | F | AGATGCACCACGAGTCTTTAC |
R | GACGTATCCACGACGGATTT |