Fig. 2

Identification of novel ‘middle-type’ KCs characterized by preferential mKast-expression. a Schematic drawing of the distribution of mKast-expressing neurons (magenta dots) in the left worker brain hemisphere. Note that the lamina of OL (the outermost layer) is absent in this illustration, which contains the entire MB structure, including the calyces and pedunculus. MB; mushroom body, AL; antennal lobe. The MB medial calyx boxed with blue line in panel (a) corresponds to both panels (b) and (c). b Nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI) of class-I KCs localized inside of an MB calyx. Dashed lines indicate boundaries of the lKCs, mKCs and sKCs. c Double-fluorescence in situ hybridization for CaMKII (green), which is preferentially expressed in the lKCs, and mKast (magenta), which is preferentially expressed in the mKCs. Nuclei of the sKCs are counter-stained with DAPI (blue). Bar, 100 μm. For panels (b) and (c), an original figure from [23], which was published in PLOS ONE, an open access journal, was reused