Table 1 Comparison of integrate efficiency among each of donor plasmids
From: An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes)
Length of homology arms | Bait sequences | Survival at 4 dpf | No GFP | GFP+ | Integrate efficiency (%) |
|---|---|---|---|---|---|
500 bp | + | 70 | 53 | 17 | 24.3 |
500 bp | - | 62 | 56 | 6 | 9.7 |
40 bp | + | 64 | 61 | 4.7 | |
20 bp | + | 110 | 108 | 2 | 1.9 |