Skip to main content
Fig. 3 | Zoological Letters

Fig. 3

From: Hox gene cluster of the ascidian, Halocynthia roretzi, reveals multiple ancient steps of cluster disintegration during ascidian evolution

Fig. 3

Mapping of Halocynthia roretzi Hox genes onto metaphase chromosomes (a-d). Metaphase chromosome spreads were prepared from cleavage stage Hr embryos and hybridized with two or three probes labeled with digoxigenin (red) or biotin (green) for genes indicated at the top of each panel. Chromosomes were stained with DAPI. Red and green arrowheads indicate signals for the gene of the same color code. In the right bottom corner of each panel, enlargement of one of the chromosomes with signals is shown in inset. Within a chromosome, a pale blue stained region corresponds to the centromeric region. The bar in d indicates 5 μm and is applicable to all panels. BAC clones used for probes were 5 J1 (Hox1), 3C14 (Hox2, Hox3 and Hox4), 6B23 (Hox5 and HoxX) and 1 J20 (Hox10, Hox11/12/13.a and Hox11/12/13.b)

Back to article page

Zoological Letters

ISSN: 2056-306X

Contact us