Fig. 2
From: Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
Generation of Tg[zhspa8:Cre-mCherry-NLS] transgenic fish and conversion of RFP transgenic fish to GFP transgenic fish. (a) A schematic of the zhspa8:Cre-mCherry-NLS plasmid. The plasmid consists of the Tol2 left arm (white box), zebrafish hspa8 promoter (zhspa8; blue box), Cre-mCherry-NLS and bovine growth hormone (BGH) polyA signal (red box), and Tol2 right arm (white box). (b) A 1dpf embryo of the Tg[hspa8:Cre-mCherry-NLS] transgenic fish. Red fluorescence derived from Cre-mCherry-NLS is present ubiquitously in the embryonic body. (c) A 9dpf larva derived from the crossing between Tg[vacht-hs:lRl-GFP] and Tg[hspa8:Cre-mCherry-NLS]. GFP instead of RFP is expressed in the trunk motoneurons (MN)