Fig. 6
From: Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
Disruption of gene function by knocking-in pnp4a. (a, b) The expression pattern of GFP in founder fish. GFP expression was observed in the eye and abdomen. These fish were considered to have “good expression” of GFP, and were raised to adulthood. GFP expression was observed in the eye and abdomen. (c–f) The progenies (4 dpf) of a pnp4a-transgenic founder crossed to an existing gu mutant. All GFP-positive F1 fish have less-pigmented eyes and all GFP-negative F1 fish have wild-type pigmentation. Asterisks indicate GFP-positive and less-pigmented F1 fish