Fig. 4

Phylogenetic comparison between piggyBac-like elements and herpesvirus-like genes (a) A Maximum-likelihood tree based on the nucleotide sequences of piggyBac-like transposase genes inside Teratorn-like viruses is shown. General time reversible model was used as the substitution model, and evolutionary rate differences among sites was modeled by discrete gamma distribution. A total of 1518 positions were used in the final dataset. b A maximum-likelihood tree based on the concatenated nucleotide sequences of five herpesvirus genes (DNA polymerase, DNA helicase, terminase, major capsid protein, membrane glycoprotein) is shown. General time reversible model was used as the substitution model, and evolutionary rate differences among sites was modeled by discrete gamma distribution. A total of 13,683 positions were used in the final dataset. c A maximum-likelihood tree based on the amino acid sequences of all annotated piggyBac-like transposase genes in teleosts is shown. Expansion of the clade surrounded by the blue square is shown on the right. Magenta indicates piggyBac-like transposase genes inside Teratorn-like viruses. JTT model was used as substitution model, and evolutionary rate differences among sites were not modeled. The bar represents the number of substitutions pre site. A total of 324 positions were used in the final dataset. Note the existence of three clusters containing piggyBac-like elements fused with Teratorn-like viruses, the phylogeny of which is highly correlated with that of Teratorn-like viruses (see Fig. 1b). Sequences used for the phylogenetic tree in (a) are marked by magenta circles. The scale bars represent the number of substitutions per site