Fig. 7
From: Ectopic Fgf signaling induces the intercalary response in developing chicken limb buds
Cellular contribution of the intercalary induced cartilage in chick limb buds. The grafted hand part was derived from a quail limb bud. a, c, e The control sample. b, d, f Fgf2 + Fgf8 bead-grafted limb. a, b Histological observation. (c–f) Immunofluorescent analysis using the antibody specific for chick cells (8F3; c, d) and for quail cells (QCPN; e, f). C’ and D’ are the higher magnification views of the boxed regions in c and d, respectively. Asterisks indicate the beads. Carp. = carpal cartilage. (G–I) The relationship between the HoxA11 expression domain and the 8F3 expression domain was investigated in the adjacent sections. g HoxA11 expression. h 8F3 antigen expression on the adjacent section. I is Hoechst staining for nuclei and is the identical section to H. The scale bars in A, C, C’, and G are 500, 500, 100, and 100 μm, respectively