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Fig. 5 | Zoological Letters

Fig. 5

From: A comprehensive comparison of sex-inducing activity in asexual worms of the planarian Dugesia ryukyuensis: the crucial sex-inducing substance appears to be present in yolk glands in Tricladida

Fig. 5

Histological analysis of test worms obtained by the administration of Fr. M0 or M10. Embedded test worms fed with either Fr. M0 or M10 were sagittally sectioned and stained with hematoxylin and eosin (HE). The dorsal sides are at the top. a–e Fr. M10 from D. ryukyuensis, (f–j) Fr. M10 from Bd. brunnea, (k–o) Fr. M0 from Bi. nobile, (p–t) Fr. M10 from T. brocchii and (u–y) Fr. M0 from A. valentianus. (a, f, k, p, and u) Domains bound by the red line are the female germ cell masses (ovaries). In, intestine. Scale bar, 100 μm. (b, g, l, q, and v) High magnification of the areas squared by the yellow line in (a, f, k, p, and u), respectively. Scale bar, 20 μm. (c, h, m, r, and w) The region indicated by the arrowheads is a developing copulatory apparatus. Ca, common antrum; Cb, copulatory bursa; Bs, bursa stalk. Scale bar, 100 μm. (d, i, n, s, and x) Domains bounded by the blue line are the male germ cell masses (testes). The cell indicated by the green arrow is a spermatid. Scale bar, 20 μm. (e, j, o, t, and y) Domains bounded by the orange-colored line are yolk glands. Scale bar, 50 μm

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Zoological Letters

ISSN: 2056-306X

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