Fig. 2

Development of the pectoral fin bud of mudskipper embryos prior to the hatching stage. Ventrolateral oblique views of mudskipper embryos (a, c, e, g) and lateral view of pectoral fin buds (b, d, f, h) at the low-pec stage (a, b; n = 1), middle-pec stage (c, d; n = 1), high-pec stage (e, f; n = 1), and long-pec stage (g, h; n = 1). Arrowheads in (a) indicate melanophores. Dorsal view of pectoral fin buds at the middle fin-fold stage (i; n = 1) and long fin-fold stage (j; n = 1). at, apical thickening; af, apical fin fold; op, optic vesicle; pec, pectoral fin. Scale bars, 100 µm in (a, c, e, g); 50 µm in (b, d, f, h, i, j)