Fig. 3

The expression maps of 12 RFamide genes in the cerebral ganglia. Open and closed circles indicate the cell bodies located in the deep and near the surface of the cerebral ganglia, respectively. Large neurons are indicated by large circles. The leftmost schemata indicate the anterior views of the brain and all the positively stained neurons are plotted. The next right two schemata show the dorsal and ventral views, and each neuron is assigned to one of these two schemata depending on the position relative to a dorso-ventral axis. The number of circles does not necessarily reflect the exact number of positive neurons, except as noted in Table 2. Numerous PC neurons expressing NP-F2 mRNA are shown in gray shade. Asymmetrically distributed neurons are circled with a red broken line for FMRFa, CCK/SK2, NP-F1, FaRP-A, and luqin1 in the schema. The photographs on the right are the representative images of in situ hybridization (coronal sections, i.e. anterior view) with antisense probes. The sectioning planes corresponding to the photographs on the right are indicated by dotted lines in the schema of the brain. Scale bar: 100 μm (applicable to all photographs). D, dorsal; V, ventral; R, right; L, left. Comments on the localization of each gene are shown in Table 2