Fig. 7

Following the same branch with DiI labeling. Two spots (a and b) on the third branch (Branch③) were labeled with DiI on Day 7. Images of the labeled branch on different days (Day 7 in a and e, Day 9 in b and f, Day 13 in c and g and Day 14 in d and h) with bright field (a-d) and with DiI fluorescence microscopy (e-h). Note that function of branch changed as development proceeds. The functional states of the branch are indicated above the images in colors. Three independent labeling experiments were performed, and the same results were obtained. Scale bars: 50 μm