Fig. 2
From: Multiple functions of non-hypophysiotropic gonadotropin releasing hormone neurons in vertebrates
Anatomical and electrophysiological characteristics of GnRH neurons in the terminal nerve and the midbrain tegmentum (a) A ventral view of the brain of gnrh3:egfp medaka embryo. GFP-expressing neurons in the terminal nerve (arrowhead) and the trigeminal nerve (arrow). b Conventional fluorescence microscopic view of the cluster of terminal nerve neurons. Bar, 20 μm. Embryos of (a) and (d) are 4~5 dpf. Yellow autofluorescence (*) originates from chromatophores of medaka body. (c) Spontaneous pacemaker activity of adult medaka (12.5 weeks after fertilization) recorded by a targeted on-cell patch clamp recording. (d) Ventral view of gnrh2:hrgfp medaka embryo. The arrowhead indicates GnRH neurons in the midbrain tegmentum (arrowheads). Dense GFP-signals (arrow) are also observed in the spinal cord. (e) Enlarged view of the cell bodies, similar to B. Bar, 20 μm. (f) Spontaneous pacemaker activity from a gnrh2:hrgfp neuron in adult medaka. e & f: modified from Kanda et al., 2010 [22]