Table 1 PCR primers used for quantitative RT-PCR and dsRNA synthesis. The primers tagged with T7 or T3 promoter sequences were used for PCR amplification for dsRNA synthesis. T7 and T3 sequences are underlined
From: The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus
Primers | Forward | Reverse |
|---|---|---|
For qPCR | ||
per | 5′-AAGCAAGCAAGCATCCTCAT-3′ | 5′-CTGAGAAAGGAGGCCACAAG-3′ |
tim | 5′-GATTATGAAGTCTGTGATGATTGG-3′ | 5′-AGCATTGGAGAGAACTGAAGAGGT-3′ |
cry2 | 5′-AGCACCATCACACACTTCACA-3′ | 5′-ACACTCAGCGCAATCCACAC-3′ |
Clk | 5′-AATGACCGTAGTCGAGAAAGTGAAG-3′ | 5′-TTGCGATGATTGAGGTTGTTG-3′ |
cyc | 5′-GGCCGAAGCTCATAAAGTGG-3′ | 5′-AACCGCACAAAGGAACCATC-3′ |
vri | 5′-TCAGCGTGGAGCAAGTGATG-3′ | 5′-GGGTACAGCAGCGAGTGTTG-3′ |
Pdp1 | 5′-TCCCGACGACAAGAAGGAG-3´ | 5′-AGCGTCTTGTCCCAGAGGTTG-3′ |
E75 | 5′-CACACGCAAGTGGAGGACA-3´ | 5′-TTTTGTGCGGCTTGTAGGC-3´ |
HR3 | 5′-CATGTTGTACCCATCAAAGGTG-3´ | 5′-TGTGGAGAGCTGGAAACTCC-3´ |
rpl18a | 5′-GCTCCGGATTACATCGTTGC-3′ | 5′-GCCAAATGCCGAAGTTCTTG-3′ |
For dsRNA synthesis | ||
per | 5′-TAATACGACTCACTATAGGGATGTGGCTTGGAAGATCATT-3′ | 5′-AATTAACCCTCACTAAAGGGTCTCCTTAAGCAAATTCTCA-3′ |
tim | 5′-AATTAACCCTCACTAAAGGGGTAAAGAAGATAGAGAGTAT-3′ | 5′-AATTAACCCTCACTAAAGGGTTGGAGAGAACTGAAGAGGT-3′ |
cry2 | 5′-TAATACGACTCACTATAGGGAAGCACACTGTGCATTGGTT-3´ | 5′-AATTAACCCTCACTAAAGGGCCGTTCTTTTCGATGATGCT-3´ |
Clk | 5′-TAATACGACTCACTATAGGGTCATAATGAGTTGAGTTCT-3′ | 5′-TAATACGACTCACTATAGGGAAGGGGTGTCTGTAATCTT-3′ |
cyc | 5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′ | 5′-AATTAACCCTCACTAAAGGGAGGTTCTGCTGCTTCTTTCG-3′ |
DsRed2 | 5′-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′ | 5′-TAATACGACTCACTATAGGGCTACAGGAACAGGTGGTGGC-3′ |