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Table 1 PCR primers used for quantitative RT-PCR and dsRNA synthesis. The primers tagged with T7 or T3 promoter sequences were used for PCR amplification for dsRNA synthesis. T7 and T3 sequences are underlined

From: The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus

Primers Forward Reverse
For qPCR
per 5′-AAGCAAGCAAGCATCCTCAT-3′ 5′-CTGAGAAAGGAGGCCACAAG-3′
tim 5′-GATTATGAAGTCTGTGATGATTGG-3′ 5′-AGCATTGGAGAGAACTGAAGAGGT-3′
cry2 5′-AGCACCATCACACACTTCACA-3′ 5′-ACACTCAGCGCAATCCACAC-3′
Clk 5′-AATGACCGTAGTCGAGAAAGTGAAG-3′ 5′-TTGCGATGATTGAGGTTGTTG-3′
cyc 5′-GGCCGAAGCTCATAAAGTGG-3′ 5′-AACCGCACAAAGGAACCATC-3′
vri 5′-TCAGCGTGGAGCAAGTGATG-3′ 5′-GGGTACAGCAGCGAGTGTTG-3′
Pdp1 5′-TCCCGACGACAAGAAGGAG-3´ 5′-AGCGTCTTGTCCCAGAGGTTG-3′
E75 5′-CACACGCAAGTGGAGGACA-3´ 5′-TTTTGTGCGGCTTGTAGGC-3´
HR3 5′-CATGTTGTACCCATCAAAGGTG-3´ 5′-TGTGGAGAGCTGGAAACTCC-3´
rpl18a 5′-GCTCCGGATTACATCGTTGC-3′ 5′-GCCAAATGCCGAAGTTCTTG-3′
For dsRNA synthesis
per 5′-TAATACGACTCACTATAGGGATGTGGCTTGGAAGATCATT-3′ 5′-AATTAACCCTCACTAAAGGGTCTCCTTAAGCAAATTCTCA-3′
tim 5′-AATTAACCCTCACTAAAGGGGTAAAGAAGATAGAGAGTAT-3′ 5′-AATTAACCCTCACTAAAGGGTTGGAGAGAACTGAAGAGGT-3′
cry2 5′-TAATACGACTCACTATAGGGAAGCACACTGTGCATTGGTT-3´ 5′-AATTAACCCTCACTAAAGGGCCGTTCTTTTCGATGATGCT-3´
Clk 5′-TAATACGACTCACTATAGGGTCATAATGAGTTGAGTTCT-3′ 5′-TAATACGACTCACTATAGGGAAGGGGTGTCTGTAATCTT-3′
cyc 5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′ 5′-AATTAACCCTCACTAAAGGGAGGTTCTGCTGCTTCTTTCG-3′
DsRed2 5′-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′ 5′-TAATACGACTCACTATAGGGCTACAGGAACAGGTGGTGGC-3′