Fig. 2

a The number of orthologous genes between C. pungelerii (Cpu) and Bambalina sp. (Bam) Among the assigned protein-coding contigs, 16,293 (Bambalina sp.) and 21,002 (C. pungelerii) genes had a TPM of 1 or higher, and the number of bidirectional best hits (BBH) between the two species was 6708. b Expression level of L/H-fibroin genes in the whole body of each individual. c Sequence alignment of the L-fibroin C-terminal region with E. variegata (Eva: GBP72856.1). d Sequence alignment of H-fibroin N/C-terminal regions and repetitive domains with E. variegata (GBP83861.1). The H-fibroin sequence was highly conserved among bagworms. The repetitive domain, represented as Rep, is divided into four motif regions: a poly-A region with glutamic acid in the centre (blue), a GA region (yellow), a linker region (light blue), and a GA region containing serine (green) as described in the box (repetitive domain architecture). From the previous SAXS analysis [12], it is predicted that the poly-A to GA region is the crystalline (β-sheet) region and the linker to GA region is the amorphous region