Fig. 2
From: Hedgehog signaling controls mouth opening in the amphioxus
Hh signaling is necessary for mouth opening in the amphioxus. a: Information on the Smo TALEN target site and the sequencing results of wild-type and Smo mutant embryos. Binding sites for the TALEN pairs [forward (Fw) and reverse (Rv)] used in this study are highlighted in gray. The BamHI site in the spacer is underlined. b: phenotypes of wild-type and Smo+/− embryos. c: phenotypes of Smo mutant embryos. The mouth present in wild-type and Smo+/− heterozygotes is lost in Smo−/− homozygous mutants, while the other pharyngeal organs, including the endostyle, club-shaped gland and first gill slit, are unaffected, m, mouth; en, endostyle; csg, club-shaped gland; fgs, first gill slit; scale bar 100 μm