Fig. 3
From: Hedgehog signaling controls mouth opening in the amphioxus
Expression of left-right regulatory genes in Smo mutant embryos. a, b: In the wild-type and Smo+/− embryos, Cer is expressed mainly in the right paraxial mesoderm. a1, b1: Cer expression is unaffected in Smo mutant embryos. c, d: Nodal exhibits an L > R pattern in WT/Smo+/− amphioxus at the early neurula stage. c1, d1: The asymmetrical expression of Nodal is unaffected in Smo mutant embryos. e, f: Lefty is expressed on the left side in WT/Smo+/− embryos at early neurula stage; e1, f1: Smo knockout has no effect on Lefty expression; g, h: Pitx is expressed on the left side of WT/Smo+/− embryos, Smo knockout has no effect on Pitx expression. Anterior to the left; L, left side; R, right side; Scale bar, 50 μm; Numbers in the top right corner of a panel show the number of times the phenotype depicted was seen out of the total number of embryos from that genotype analyzed