Fig. 6
From: Hedgehog signaling controls mouth opening in the amphioxus
Pou4 expression during mouth development in the amphioxus. Images of B. floridae at the tail bud stage (T1 stage) and the open mouth stage (L0 stage) from the left lateral view. Black arrows mark the mouth region, scale bar, 100 μm. a, a1: Pou4 is expressed focally in the regions destined to form the mouth (a), Smo gene knockout has no effect on Pou4 expression (a1). b, b1: Pou4 is expressed at the margin of the mouth at the L0 stage. Smo knockout results in diminished Pou4 expression at the mouth margin with the complete loss of mouth opening