Fig. 2
From: An approach for elucidating dermal fibroblast dedifferentiation in amphibian limb regeneration
The cultured newt cells were grafted into regenerating axolotl limb blastema. A The schematic diagram of the experiment. B Just after the grafting. Scale bar in B1 = 1 mm. C 10 days after the grafting. Newt cells (red) were spread out in the regenerate (insert). D–F The distribution of the newt cells was investigated on the section. Immunofluorescent analysis for Col2a (green; D1 and E1) and mCherry (red; D2 and E2) was performed. Nuclei were visualized by Hoechst33342 (blue; D3 and E3). F the merged image of the boxed region in E1 and E2. The red signals were observable within the Col2a + region. The scales bar in D1 and F are 300 and 100 μm, respectively