Table 4 Primers used in qPCR of medaka Cry genes
Gene | Primer | Sequence (5’ to 3’) | Amp. Eff. (%) |
|---|---|---|---|
OlCry1aa | Forward | TGTCC TGCCG CCTCT TTTAC | 108.5 |
Reverse | AAAAG AATTC ACGCC ACAGC AG | ||
OlCry1ab | Forward | ACCCT GCGCT GCATC TAC | 113.2 |
Reverse | CCTCG GATGA CGAAC AAGC | ||
OlCry1ba | Forward | CAACA TCGAG AGGAT GAAGC AG | 91.4 |
Reverse | TCCTC CATTC CCGTT CCCAT | ||
OlCry2 | Forward | TATTC TGGAC CCCTG GTTCG | 102.8 |
Reverse | CCTGG AGTTG AGCTT CTTCA G | ||
OlEf1α | Forward | TCTAC AAGTG CGGAG GAATC G | 94.7 |
Reverse | GTCCA ACACC CAGGC GTACT | ||
OlHprt1 | Forward | ACCGC TCCAT TCCCA TGAC | 98.0 |
Reverse | TGCCG GTTAG GGTAG ACAGG | ||
OlGusb | Forward | TGACC CACGA GAATC CAGGT | 99.3 |
Reverse | CGGAT GCCAA CAGGA AGAGT |