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Table 4 Primers used in qPCR of medaka Cry genes

From: Midnight/midday-synchronized expression of cryptochrome genes in the eyes of three teleost species, zebrafish, goldfish, and medaka

Gene Primer Sequence (5’ to 3’) Amp. Eff. (%)
OlCry1aa Forward TGTCC TGCCG CCTCT TTTAC 108.5
Reverse AAAAG AATTC ACGCC ACAGC AG
OlCry1ab Forward ACCCT GCGCT GCATC TAC 113.2
Reverse CCTCG GATGA CGAAC AAGC
OlCry1ba Forward CAACA TCGAG AGGAT GAAGC AG 91.4
Reverse TCCTC CATTC CCGTT CCCAT
OlCry2 Forward TATTC TGGAC CCCTG GTTCG 102.8
Reverse CCTGG AGTTG AGCTT CTTCA G
OlEf1α Forward TCTAC AAGTG CGGAG GAATC G 94.7
Reverse GTCCA ACACC CAGGC GTACT
OlHprt1 Forward ACCGC TCCAT TCCCA TGAC 98.0
Reverse TGCCG GTTAG GGTAG ACAGG
OlGusb Forward TGACC CACGA GAATC CAGGT 99.3
Reverse CGGAT GCCAA CAGGA AGAGT