Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs40851-022-00194-2/MediaObjects/40851_2022_194_Fig5_HTML.png)
Specificity of the mZPC5 antibody and detection of mZPC5 in female tissue extracts. SDS-PAGE results are shown for liver (A) and ovary (B) tissue extracts of spawning females. A: the 10% SDS-PAGE pattern of CBB-stained proteins in the female liver extracts (Aa) and the associated proteins that were immunoreactive to the anti-mZPC5 antibody (Ab). B: the 8% SDS-PAGE pattern of CBB-stained proteins in the female ovary extracts (Ba) and the associated proteins that were immunoreactive to the anti-mZPC5 (Bb), anti-Chg.L (Bc), and anti-Chg.H (Bd) antibodies, respectively. Symbols – yellow star: ~ 74-kDa proteins reactive to the anti-mZPC5 anyibody, black arrow: ~ 50-kDa proteins reactive to the anti-mZPC5 antibody