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Fig. 5 | Zoological Letters

Fig. 5

From: Medaka, Oryzias latipes, egg envelopes are created by ovarian-expressed ZP proteins and liver-expressed choriogenins

Fig. 5

Specificity of the mZPC5 antibody and detection of mZPC5 in female tissue extracts. SDS-PAGE results are shown for liver (A) and ovary (B) tissue extracts of spawning females. A: the 10% SDS-PAGE pattern of CBB-stained proteins in the female liver extracts (Aa) and the associated proteins that were immunoreactive to the anti-mZPC5 antibody (Ab). B: the 8% SDS-PAGE pattern of CBB-stained proteins in the female ovary extracts (Ba) and the associated proteins that were immunoreactive to the anti-mZPC5 (Bb), anti-Chg.L (Bc), and anti-Chg.H (Bd) antibodies, respectively. Symbols – yellow star: ~ 74-kDa proteins reactive to the anti-mZPC5 anyibody, black arrow: ~ 50-kDa proteins reactive to the anti-mZPC5 antibody

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Zoological Letters

ISSN: 2056-306X

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