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Fig. 6 | Zoological Letters

Fig. 6

From: Medaka, Oryzias latipes, egg envelopes are created by ovarian-expressed ZP proteins and liver-expressed choriogenins

Fig. 6

Immunohistochemical detection of mZPC5 in the ovaries using anti-mZPC5 and anti-Chg.H antibody. All images in the same row show the same tissue section. The first three columns (left to right) show the tissues under different microscope light settings, while the last column shows images from columns one, two and three overlaid. A, E, and I: transmitted light images of oocytes in the ovaries of sexually mature females. B, F, and K: the portions of the tissues reacted with the anti-mZPC5 antibody (green). C, G, and L: the parts of the tissues reacted with the anti-Chg.H (positive control) antibody (red) in the same ovarian oocyte as A, E, and I, respectively. The white-circled area in Fig. 6A was magnified 2.5 times in Fig. 6E–H to visualize structural details in the developing chorion and ovarian oocyte. Symbols – y: yolk, yellow arrows: the position of the chorion, white arrows: early developed oocytes. Scale bars in A, E, and I represent 100, 40, and 100 µm, respectively. The developing stage of oocytes was determined following guidance from Iwamatsu et al., 1988 [34]

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