Fig. 1
From: Air–breathing behavior underlies the cell death in limbs of Rana pirica tadpoles
Air-breathing behavior, and cell death during limb development in R. pirica. a Air–breathing behavior of R. pirica tadpoles. b A lung dissected from a stage 36 R. pirica tadpole. c Cell death (LysoTracker Red) in R. pirica hindlimbs. LysoTracker–positive cells were found in interdigital regions, and limb margin edges (arrowheads), and surface ectoderm (arrows). d Cell death (LysoTracker Red) in mid stage 36 R. pirica hindlimbs. Cell death was observed in both the ectoderm (arrows) and the mesenchyme (arrowheads), as revealed on the orthogonal (optical) section of a confocal stack. D2, 4–5: digits 2, 4, and 5; ID3, 4: interdigital regions 3 and 4. Scale bars, 500 μm in (b), (c) and 100 μm in (d)