Table 2 Primers for V1Rs
From: Type 1 vomeronasal receptor expression in juvenile and adult lungfish olfactory organ
Probe name | Target V1R gene | Product size (bp) | Forward primer (5 > 3) | Reverse primer (5 > 3) |
|---|---|---|---|---|
Paeth01 | P. aethiopicus V1R23 (ancV1R) | 597 | CCCACAGTTAGCTGGCGTAA | GGTTTTGGCATGCCTCATGG |
Paeth02 | P. aethiopicus V1R52 | 403 | CATTGGTTTGACCTGCCTGC | CTCTGCTCCAGCTTCCTGAC |
Paeth03 | P. aethiopicus V1R53 | 566 | AGCCTAGCATGCTCAAACCT | ACCACCATCTTGGATGCCTG |
Paeth04 | P. aethiopicusV1R55 | 551 | TGCTGTTGGCCTTGCAAGTA | TTGCCACAGCCATAAGGACT |
Paeth05 | P. aethiopicusV1R69 | 599 | TGCTAAGCTGCTTCCAGTGT | AGAGTGGCAAGTCACTGCAT |
Paeth06 | P. aethiopicusV1R71 | 658 | CTTCTGACTGGGGGTGTTCC | CCAAGGACAGAAAATGCCGC |
Paeth07 | P. aethiopicusV1R83 | 596 | ACTTGCCAACCCACCAAGAA | GAAATGCAACGTCACGAGCA |
Paeth08 | P. aethiopicusV1R94 | 563 | CGTGTTTGTCGAGCGATGTC | GCAAAGAAGACACGGGCATC |
Paeth09 | P. aethiopicusV1R103 | 545 | CTTTTCACGCTGGGACTTCC | GTGACAACAGTCTTGGCAGC |
Paeth10 | P. aethiopicusV1R111 | 543 | GGGGCAAACCTGTTACTCCT | TGCTTGTAGCTCTGCTGTGG |
Paeth11 | P. aethiopicusV1R116 | 523 | CGAGAGGCATTCCTGAACCA | TTAGCTGCCTGACCTTCTGC |
Paeth12 | P. aethiopicusV1R119 | 402 | GACAAGTACTGGTGTTCTGGGT | TAAAGGAGCAGGCCACAACA |
Paeth13 | P. aethiopicusV1R136 | 477 | TGATCCTTTGCAACCTGGGA | ACAAAATGTTGCTGCTGGCC |
Paeth14 | P. aethiopicusV1R140 | 589 | CCGTGTTTTTCGAGCGATGT | AGGACTGACAGCAGCATACA |
Paeth15 | P. aethiopicusV1R141 | 513 | CCAGAGGAATGCCACAGACA | CCCTGGCTTCAGCTGAAACA |
Paeth16 | P. aethiopicusV1R144 | 432 | CACTGAACTGGCAGGGACAA | ATCAGGTCACGGGCAAAACT |
Paeth17 | P. aethiopicusV1R148 | 645 | TCAGAGCTGTCAGTGGCAAA | CCGTGACACTGATGCCTGAT |
Paeth18 | P. aethiopicusV1R159 | 393 | CAAGTACTGGAGTCTTGGGCA | GCAGGCCACAATGCATAACC |
Paeth19 | P. aethiopicusV1R160 | 695 | TGGAAACATCACATCCGGCA | TGCTTGCTTCTCTGCTGTGA |
Paeth20 | P. aethiopicusV1R166 | 525 | TACCCGAGGTCTTCCAGCAA | GCTGCTTTCACCTCTACAGC |
Paeth21 | P. aethiopicusV1R198 | 410 | GTAGTAAGCGGCATCCCTGG | ACAGTGTACATTGGTGGGCT |
Paeth22 | P. aethiopicusV1R208 | 527 | GGTTGTGCTGACAGTAGGCA | CTTGGGCTTCTGCACTGTTC |
Paeth23 | P. aethiopicusV1R213 | 549 | ATGGTTGCTTTGCTGTCACG | TACAACCGACTTTGCAGCCT |
Paeth24 | P. aethiopicusV1R218 | 539 | AGCTTCACAAAAGGGGCCAT | GCAAAGCCGTTCACCTGAAA |
Paeth25 | P. aethiopicusV1R227 | 520 | CAAGAGGGGTTCCAGACTGT | GCTGCTCTGTTCTCTGCTGT |
Paeth26 | P. aethiopicusV1R257 | 305 | CTCTGTGTGCTTGCTATGGC | ACTGTTTTTGCTGCTTGGCC |
LP01 | L. paradoxa V1R20 (ancV1R) | 599 | TACTGTTAGCTGGCGCAACA | TCTGCGTTTGGGGATTCCTC |
LP02 | L. paradoxa V1R59/L. paradoxa V1R60 | 620 | AATGAGCTGCCCCAAACTGA | AGGTGACAACAGTTCGCGTA |
LP03 | L. paradoxa V1R64 | 640 | ACAGTTACTGGAGCTGTGGG | TCTCTGCACTGTTCTCCAGC |
LP04 | L. paradoxa V1R65 | 543 | ACCTGTCAACAGCAAACCTGA | TTGCTGCTTGACTCTCTGCA |
LP05 | L. paradoxa V1R70 | 525 | TGCAAGAGGAGTGCCACAAA | GATTTTGCTGCCCTGGCTTC |
LP06 | L. paradoxa V1R80 | 552 | ACCAGCAAACCTCACCATCA | ATGTAGCTGCTGGCAAGTGT |
LP07 | L. paradoxa V1R89 | 554 | TTGCTGTCCGGAGTAAACCT | GCTGCTTGGCTTTCTGCATT |
LP08 | L. paradoxa V1R92/L. paradoxa V1R93 | 490 | GGATCAGTGTCCTGGACAGC | TGAGGTCACGGCCAAAAAGA |
LP09 | L. paradoxa V1R99 | 652 | CAGGTCTCTCTGGGGACTGA | AGGCAAAGTGTTGAGGCAGT |
LP10 | L. paradoxa V1R103/L. paradoxa V1R104 | 510 | ACTTGGCCATCACTGGATCC | CCACCATGAGATCTCGGCTG |
LP11 | L. paradoxa V1R120/L. paradoxa V1R121 | 439 | TCACATCCCACCTTGCTTTT | ATTACAGCATCACGCCCTGT |
LP12 | L. paradoxa V1R127 | 707 | CTGCCCATGGTCTTCTCCAA | AATGGGGTCTCACCTGTTGC |
LP13 | L. paradoxa V1R130 | 556 | TCCTGCCAACATTGCCATCT | AAAAAGGATTGCTGCGCTGG |
LP14 | L. paradoxa V1R139 | 412 | TATCACGCGGCATGGCTATT | GACTCGGTGCGATCCTTCAT |
LP15 | L. paradoxa V1R142 | 524 | AGTGTGTGAGTGTCAGTGCA | TGCAGCATAGCACATCGAGA |
LP16 | L. paradoxa V1R172 | 540 | GAGCTGCTTCCAGTATGCCA | ACTGAAGCATAGCACGTGGA |