Fig. 5

Hatching instar 1 of Nymphon micronesicum. A Lateral view of adult male bearing embryos and hatched instars; stereomicroscopic autofluorescence image. White arrowheads point to the ovoid-shaped hatching instar 1. B Instar 1 after Sytox staining, epifluorescence image. C and D Ventral and lateral views, respectively. CLSM scans (blend mode) of cuticular autofluorescence (magenta) and Sytox staining (gray) shown separately. White arrows indicate the scape boundaries. Small white arrowheads trace fibrous secretions projecting from the attachment gland spine. Black arrowheads point to the putative area of the attachment gland’s reservoir cells. The ovals mark the incipient oviger bud. The red stippled outline exemplarily highlights leg 1 that is folded and partially hidden under the cuticular cover. The double arrow points to the small bud of leg 4. E Virtual parasagittal section (Imaris oblique slicers), CLSM scan of autofluorescence (magenta) and Sytox staining (gray). The arrows mark borders between ventral ganglia. The green stars lie next to ventral organ cavities/invaginations. F&G Virtual horizontal sections (Imaris oblique slicers) of the posterior body pole, CLSM scans of Sytox staining. F The red outline marks leg 3 of one body half. The green stippled lines highlight the ganglion anlagen in the other body half. The primordial invagination of the ultimate ventral organ (green star) is visible posteriorly. G The proctodeum (arrowhead) forms at the tip of the anal tubercle. Abbreviations: ags – attachment gland spine; br – brain; ch – cheliphore; l1–3 – leg pairs 1–3; lg1–4 – leg ganglia 1–4; pa – palp; pg – posterior ganglion; pr – proboscis; sc – scape; seg – subesophageal ganglion; yo—yolk