Fig. 6

The gastrovascular canal of V. multiformis. a A schematic representation indicating positions of the following images. b The gastrovascular canal of V. multiformis. Radiating canals with multiple branches were observed with phalloidin staining. c A gastrovascular canal was stained with an anti-α-tubulin antibody, phalloidin and DAPI. Arrowheads indicate the ciliated rosette. d-d’’’ A series of images from external (d) to internal (d’’’) focal planes of one ciliated rosette. e The lateral view image of the two ciliated rosettes. f The schematic drawings of the ciliated rosette reconstructed from the immunofluorescent images. g A magnified view of the canal. α-tubulin-positive cilia covered the inner surface. Colors in all images except b indicate anti-α-tubulin (green), phalloidin (magenta), and DAPI (blue) fluorescence. Scale bar, 20 μm (b, c and g) or 5 μm (d, e)