Fig. 2

Expression pattern of Ppa-nhr-10 across tissues. A 1.371 kb-long promoter sequence of Ppa-nhr-10 (ending immediately before the start codon) was fused to P. pacificus-optimized GFP. Relative strength of fluorescent-protein expression is indicated via a relative intensity scale. (A) and (B) each show a representative worm from two independent reporter lines in different orientations: (A) right body side facing reader, (B) left body side facing reader. Asterisk (*) in (A) indicates the position of anterior gonad arm. Note that both, anterior and posterior gonad arms, are always reporting; absence of the anterior arm in (A) is due to image stacking only. The crossed double-arrows with letters in (A) and (B) indicate anatomical orientation of the worm: A = anterior, P = posterior, D = dorsal, V = ventral. (C) Zoom-in on the head of the worm depicted in (B), merged with DIC image. (D) Zoom-in on the anterior gonad arm (outlined with white dots) of the worm depicted in (B). Note that neither the sperm cells nor the eggs express Ppa-nhr-10; only the somatic gonad tissues do so. amp = ampulla of the gland cell(s), g1D = dorsal pharyngeal gland, g1V = ventral pharyngeal glands, sp = sperm